Detection of leptospiral DNA by PCR
نویسندگان
چکیده
منابع مشابه
Detection of leptospiral DNA by PCR.
An EcoRI fragment (1.2 kb) which is highly conserved among Leptospira interrogans isolated in Korea was cloned into pBluescript vector from L. interrogans serovar lai WH20. The EcoRI fragment was sequenced, and a pair of primers (LP1 and LP2) was designed for PCR assay. PCR amplification of target DNA obtained from cultured L. interrogans showed that 274 bp could be detected when as little as 1...
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Specific detection of Brucella DNA by PCR.
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متن کاملmolecular detection of pathogenic leptospiral serovars by pcr, based on lipl21 gene
leptospirosis is a zoonotic disease with global distribution that caused by pathogenic spirochetes of the genus leptospira. accurate diagnosis for differentiation of leptospirosis from other pyrogenic infections prevailing in the same locality and is imperative for proper treatment. therefore a molecular diagnostic test with high specificity and sensitivity such as pcr is essential. gene encodi...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1994
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.32.4.1035-1039.1994